Anionic Self?Assembling Supramolecular Enhancers of Antimicrobial Efficacy against Gram?Negative Bacteria

As a result of the looming antimicrobial resistance crisis, there is an urgent need for novel treatments. This particularly true hard-to-treat Gram-negative bacteria, as many agents are unable to cross cell membrane gain access interior, and thus elicit therapeutic response. Herein, evidence provided use anionic supramolecular self-associating amphiphiles (SSAs) efficacy enhancers commonly used agents, which known resistance, against bacteria. The co-administration SSAs with antimicrobials shown sensitize traditionally hard treat Pseudomonas aeruginosa both rifampicin novobiocin, from structure activity relationships can be elucidated. Quantitative fluorescence microscopy performed, indicating permeabilization likely mode action drug enhancement by SSAs. These results offer alternative strategy in adjuvant design, expanding focus beyond cationic peptides into realm small molecules. Finally, self-assembly presence these investigated through combination quantitative NMR, tensiometry, dynamic light scattering, zeta potential studies, demonstrating impact on SSA self-association events.